Agile SciTech
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Digital PCR & molecular quantification

Count DNA and RNA molecules directly — no standard curve. For residual DNA, rare mutations, copy number and pathogen detection, with spatial RNA detection in tissue alongside.

Who uses it

Molecular biology and oncology research labs, biopharma QC, gene therapy developers and hospital research groups.

Partners in this workflow
RainSureBio-Techne

Challenges we help solve

Challenge 1qPCR variability

Standard curves add error and make results hard to compare between labs.

Challenge 2Rare targets in high background

Low-frequency mutations and trace DNA are below qPCR detection limits.

Challenge 3Hands-on time

Multi-instrument dPCR workflows need skilled operators and long setup.

The workflow, step by step

  1. 1Assay selection

    Choose a ready kit or transfer an existing qPCR assay.

    RainSure dPCR assays · master mix
  2. 2Partition & amplify

    Split each sample into thousands of reactions.

    RainSure RS32 · DropDx · OmniPlex
  3. 3Absolute quantification

    Read positive partitions to count copies directly.

    RainSure analysis software
  4. 4Spatial confirmation

    See where target RNA is expressed in tissue.

    Bio-Techne RNAscope™ ISH

Recommended products

7 products for this workflow. Click a picture for a quick view, or add products to your quote list.

Quote the whole workflow

Tell us your targets and sample type.

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