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Downstream purification

Capture, polish, concentrate and buffer-exchange proteins, antibodies, peptides and oligos — with chromatography systems, prep HPLC, TFF and columns that scale from bench to plant.

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Who uses it

Process development and manufacturing teams purifying mAbs, recombinant proteins, peptides and oligonucleotides.

Partners in this workflow
InscinstechWelch MaterialsUnchained Labs

Challenges we help solve

Challenge 1Scale-up without re-validation

Methods developed on lab FPLC need a clear path to pilot and production systems.

Challenge 2Yield vs purity trade-offs

Polishing steps must remove aggregates and impurities without losing product.

Challenge 3Fast in-process checks

Concentration and aggregation need checking between steps, quickly and with little sample.

The workflow, step by step

  1. 1Capture

    Bind and elute the target from clarified harvest.

    Welch Protein A / IEX resins · Inscinstech FPLC
  2. 2Polishing

    Remove aggregates, variants and impurities.

    Inscinstech prep HPLC · Welch prep columns
  3. 3Concentration & buffer exchange

    Bring product to formulation buffer and concentration.

    Inscinstech TFF · Unchained Lil’ Tuna
  4. 4In-process analytics

    Check concentration and size between steps.

    Unchained Lunatic, Stunner

Recommended products

9 products for this workflow. Click a picture for a quick view, or add products to your quote list.

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Related applications

Upstream cell culture Oligo, mRNA & peptide manufacturing Biologics developability